Phosphorus (P) enrichment and human activity in fluvial ecosystems: P dynamics, microbial biomarkers and remediation strategies

Project CEAB

FLUVIAL-PREMOVAL

Fluvial-Premoval, aims on providing a set of tools for managing and mitigating the effects of Phosphorous pollution in stream ecosystems which is, indeed, among the major and more persistent impacts for the surficial water quality worldwide.

The project integrates an innovative transdisciplinary approach, combining bioengineering techniques, manipulative experiments in mesocosms and surveys in the real-world (Ter) and the characterization of the microbiota associated to biofilms in rivers.

Fluvial-Premoval proposes an integrative approach combining principles and methods of advanced research from different but complementary research areas (fluvial biogeochemistry, microbial ecotoxicomics and restoration ecology). The also integrates the collaboration with other research groups, companies and other stakeholders social actors interested in this environmental and water quality problem.

The central aim of this project is to gain insights about the factors and mechanisms controlling P dynamics in fluvial ecosystems subjected to human pressures and examine how microbial communities in fluvial biofilms integrate integrate the different impacts associated to P availability.

The ultimate goal is to provide knowledge and a specific tool set to stream managers to assess problems associated with high P availability and to help mitigation in stream and river ecosystems.

The project is organized in two work packages (WP). In WP1 we will characterize the presence of the different P forms in the Ter river, along a gradient of human impact. We will describe structural and functional attributes of biofilms by performing molecular analysis of the microbial community. In this WP we will also assess, differences in bioavailability between dissolved and particulate P under controlled experimental conditions (indoor channels). Similarly, we will also test differences between organic and inorganic P and its destination and effects on biofilms.

These experiments will contribute to understand the trends observed in real-world and confirm the relationship between P availability and biofilm responses.

WP2 will evaluate how the hydromorphology of streams (factor determining habitat configuration and hydrologic retention) and changes in the dissolved inorganic nitrogen and dissolved organic carbon (determining biotic activity) affect the amount of P retained in streams.

Tasks included in WP2 will be conducted in the Urban River Lab experimental platform (www.urbanriverlab.com) and in a 360-m reconstructed stream by bioengineering within three 80-m reaches.

 

We will also evaluate how the hydromorphlogical characteristics of a reach, as a factor modifying hydrologic retention and habitat configuration, and changes in the relative availability of dissolved inorganic nitrogen and dissolved organic carbon, as a factor influencing biotic activity, can influence P retention at reach-scale in a receiving stream.

We will also investigate the role of biofilms in P uptake in each reach and assess how the reciprocal interactions between P availability and the structural and functional attributes of the biofilm associated with P cycling vary among different habitats. Variability in biofilm P markers observed at habitat scale within reaches will then be compared to that observed at larger spatial scale in WP1.

This comparison will allow expanding knowledge on the use of biofilm P markers across observational scales.

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Bernal S, Lupon A, Ribot M, Sabater F, Martí E (2015). Riparian and in-stream controls on nutrient concentrations and fluxes in a headwater forested stream. Biogeosciences 12: 1941-1954. https://doi.org/10.5194/bg-12-1941-2015

PDF. Bernal S, Lupon A, Wollheim WM, Sabater F, Poblador S, Martí E (2019). Supply, demand, and in-stream retention of dissolved organic carbon and nitrate during storms in Mediterranean forested headwater streams. Frontiers in Enviornmental Science 7: 60. https://doi.org/10.3389/fenvs.2019.00060

Bussi G, Whitehead PG, Gutiérrez-Cánovas C, Ledesma JLJ, Ormerod SJ, Couture R (2018). Modelling climate and land-use change impacts on nitrate and aquatic ecosystems in the River Wye (Wales). Science of the Total Environment 627: 733-743. https://doi.org/10.1016/j.scitotenv.2018.01.295

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Gacia E, Bernal S, Nikolakopoulou M, Carreras E, Morgado L, Ribot M, Isnard M, Sorolla A, Sabater F, Martí E (2019). The role of helophyte species on nitrogen and phosphorus retention from wastewater treatment plant efluents. Journal of Environmental Management 52: 109585. https://doi.org/10.1016/j.jenvman.2019.109585

Gomez N, Donato JC, Giorgi A, Guasch H, Mateo P, Sabater S (2009). La biota de los ríos: los microorganismos autótrofos. Conceptos y técnicas en ecología fluvial. Editors: Arturo Elosegi i Sergi Sabater, capítol 12, p. 219-242. ISBN: 978-84-96515-87-1. http://hdl.handle.net/10256/10259

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Lannergård E, Ledesma JLJ, Fölster J, Futter MN (2019). An evaluation of high frequency turbidity as a proxy for riverine total phosphorus concentrations. Science of the Total Environment 651: 103-113. https://doi.org/10.1016/j.scitotenv.2018.09.127

Lupon A, Martí E, Sabater F, Bernal S (2016). Green light: gross primary production influences seasonal stream N export by controlling fine‐scale N dynamics. Ecology 97(1): 133-144. https://doi.org/10.1890/14-2296.1

Lupon A, Denfeld BA, Laudon H, Leach J, Karlsson J, Sponseller RA (2019). Groundwater inflows control patterns and sources of greenhouse gas emissions from streams. Limnology and Oceanography 64(4): 1545-1557. https://doi.org/10.1002/lno.11134

Lupon A, Catalán N, Martí E, Bernal S (2020). Influence of dissolved organic matter sources on in-stream net dissolved organic carbon uptake in a Mediterranean stream. Water 12(6): 1722. https://doi.org/10.3390/w12061722

PDF. Martí E, Feijoó C, Vilches C, Ferreiro N, Gantes P, Ranieri C, Torremorell A, Rodríguez-Castro MC, Gultemiriam ML, Giorgi A, Sabater F (2020). Diel variations of nutrient retention and metabolism are coupled for ammonium but not for phosphorus in a lowland stream. Freshwater Science 39(2): 268-280. https://doi.org/10.1086/708933

Nikolakopoulou M, Argerich A, Drummond JD, Gacia E, Martí E, Sorolla A, Sabater F (2018). Emergent Macrophyte Root Architecture Controls Subsurface Solute Transport. Water Resources Research 54(9): 5958– 5972. https://doi.org/10.1029/2017WR022381

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Ribot M, von Schiller D, Martí E (2017). Understanding pathways of dissimilatory and assimilatory dissolved inorganic nitrogen uptake in streams. Limnology and Oceanography 62(3): 1166-1183. https://doi.org/10.1002/lno.10493

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PDF. Tank JL, Martí E, Riis T, Von Schiller D, Reisinger AJ, Dodds WK, Whiles MR, Ashkenas LR, Bowden WB, Collins SM, Crenshaw CL, Crowl TA, Griffiths NA, Grimm NB, Hamilton SK, Johnson SL, McDowell WH, Norman BM, Rosi EJ, Simon KS, Thomas SA, Webster JR (2018). Partitioning assimilatory nitrogen uptake in streams: an analysis of stable isotope tracer additions across continents. Ecological Monographs 88(1): 120-138. https://doi.org/10.1002/ecm.1280

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Von Schiller D, Martí E, Riera JL, Ribot M, Argerich A, Fonollà P, Sabater F (2008). Inter-annual, Annual, and Seasonal Variation of P and N Retention in a Perennial and an Intermittent Stream. Ecosystems 11(5): 670–687. https://doi.org/10.1007/s10021-008-9150-3

GUASCH, HELENA

CEAB-CSIC

IP del projecte (WP1)

Experta en ecotoxicologia de rius i ha estat IP en diversos projectes analitzant els efectes ecològics dels productes químics sobre els biofilms, macroinvertebrats i peixos.

MARTÍ, EUGÈNIA

CEAB-CSIC

IP del projecte (WP2) i de l’Urban River Lab

Experta en ecologia i biogeoquímica d’ecosistemes fluvials i respostes en front al canvi global, tant en sistemes verges com urbans.

ROMANÍ, ANNA MARIA

Institut d’Ecologia Aquàtica, UdG

Experta en ecologia de biofilms microbians

GACIA, ESPERANÇA

CEAB-CSIC

Experta en ecologia funcional de comunitats dominades per plantes aquàtiques i en l’ús dels macròfits com a bio-indicadors de canvis. Interès en restauració i conservació d’ecosistemes aquàtics.

BERNAL, SUSANA

CEAB-CSIC

Experta en hidrologia i cicles biogeoquímics en la interfase riu-ribera i a escala de conca hidrogràfica.

RIBOT, MIQUEL

CEAB-CSIC

Expert en ecologia i biogeoquímica fluvial, amb experiència en tècniques per a caracteritzar el ciclat de nutrients als rius.

PROIA, LORENZO

BETA Technologial Center, UVic

Expert en ecologia microbiana dels biofilms fluvials i la seva capacitat de captació de nutrients.

LUPON, ANNA

CEAB-CSIC

Experta en el cicle del carboni i metabolisme en rius i interaccions biogeoquímiques riu-ribera.

LEDESMA, JOSÉ L. J.

Karlsruhe Institute of Technology (KIT)

Expert en hidrologia de conca i movilització de nutrients.

ARGUDO, MARIA

CEAB-CSIC

Experta en ecotoxicologia microbiana, creació de bases de dades, disseny de mostreig, anàlisi de dades físicoquímics i biofilm, anàlisi bioinformàtic i bioestadístic.

LÓPEZ, ALEJANDRO

Institut d’Ecologia Aquàtica, UdG

Ecologia i conservació aqüàtica, tractant com els impactes antropogènics afecten a biofilms, macroinvertebrats, peixos y amfibis.

BOLIVAR, MANEL

CEAB-CSIC

Ajudant de camp.

OLIVER, ANNA

Associació Mediterranean Eddy 4.0

Experta en comunicació i divulgació científica.

MERBT, STEPHANIE N.

EAWAG Aquatic Research

Experta en anàlisis metatranscriptòmics i metagenòmics microbians.

THOMAS, STEVEN

Universitat de Nebraska

Ha participat en els desenvolupaments teòrics i experimentals amb múltiples nutrients amb els membres del CEAB.